I have a site that needs the following in there .htacess file: Options All -Indexes DirectoryIndex index.php index.htm index.html <Files .ftaccess> deny from all </Files> <Files .staccess> deny from all </Files> Everytime there .htacess file is updated with this information so pages can be accessed. Once the .htacess is emptied everything starts to work fine.
You might have to change the AllowOverride setting of the appropriate directory at the end of your main Apache configuration (in the part that the ISPConfig installer wrote). Have a look here: http://www.howtoforge.com/forums/showthread.php?t=5872