The config is overridden by the ispconfig.conf file in /etc/apache2/sites-available. Commenting the rules in that file for /usr/share/phpmyadmin out worked for me, but it is not a good idea as this config will be overwritten when updating ISPConfig. You can prevent this by adding the changed files as conf-custom file, but I wouldn't do that as that could break your system if we change something important in that file. So, I had to look into it further. I got it working by adding Code: AllowOverride AuthConfig right beneath Code: <Directory /usr/share/phpmyadmin> in /etc/apache2/conf-available/phpmyadmin.conf, and adding the lines I needed for auth to a .htaccess in /usr/share/phpmyadmin/.htaccess (Remember to restart apache after modifying the conf file)